UPLC / HPLC / LC

The Liquid chromatography (LC) is an analytical instrument used in liquid chromatography (LC) to perform the separation, identification, and quantification of components within a sample. And it is a high accurate detector to detect specific materials might existing in a sample liquid. Such as nanomolar or even picomolar quantities of various analytes ranging from drugs, drug and food metabolites, biomarkers.  So the Liquid chromatography can be used for analytical or preparative applications. It is an analytical technique used to separate the chemical components of a sample mixture and then detect them to determine their presence or absence.

High performance liquid chromatography (HPLC) is a method in chemistry used to split different substances in a mix. This process works by pushing a liquid mix through a tube filled with another substance. As the mix travels, its parts separate because they interact differently with the substance in the tube.

A tool called a detector checks the parts once they come out of the tube, and a system called a chromatography data system (CDS) reads the signal the detector sends.

The result of a high performance liquid chromatography test is shown in a chart called a chromatogram. On this chart, time is shown on the horizontal line, and the signal from the detector is shown on the vertical line.

UPLC stands for Ultra Performance Liquid Chromatography. It's a newer and improved version of HPLC that's become the standard nowadays. UPLC is better because it helps process samples faster, works more efficiently in separating substances, is more sensitive in detecting components, and makes the time it takes for a run shorter. Also, a UPLC machine can manage pressures up to 15,000 pounds per square inch (psi).